Methods in Molecular Medicine32- Alzheimer's Disease

Nigel M Hooper

• 09 november 2010
• 9781617371615

Samenvatting:

The aim of Alzheimer's Disease: Methods and Protocols is to bring together the main biochemical, cell biological, and molecular biological techniques and approaches that are being used to investigate the molecular basis of Alzheimer's disease.

With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert who uses it on a day-to-day basis. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagants and of amplification systems. Also discussed are the use of animal models to understand human muscular dystrophy and the available options for gene-based therapy.
Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today‚s diagnostic laboratories, basic and medical researchers, and active clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge.


A panel of highly skilled investigators describe in detail their state-of-the-art biochemical, cell biological, and molecular biological techniques for studying the molecular basis of Alzheimer's disease. These readily reproducible, step-by-step methods focus on work with the amyloid precursor protein and the amyloidogenic peptide Ass, but also include-with the recent identification of presenilin proteins-techniques for determining the structure and biological function of these proteins. In addition, there are chapters covering the tau protein and its role in Alzheimer's disease, as well as an introductory discussion of the history of the disease, its genetic basis, and the currently available and possible future therapeutic agents. Cutting-edge and wide ranging, Alzheimer's Disease: Methods and Protocols provides ready access to proven, reproducible methods for elucidating the molecular basis of this most common senile dementia.